Code to reproduce the analyses of the AWST paper: MIXOLOGY
S.M.Pagnotta - Dept. of Science and Technology, Universita’ degli Studi del Sannio (Benevento, Italy) D.Risso - Dept. of Statistical Sciences, Universita’ degli Studi di Padova (Padova, Italy)
23 September, 2020
blah blah
This file belongs to the repository: https://github.com/drisso/awst_analysis.
The code is released with license GPL v3.0.
(Note: I don’t think we can release these images with GPL license, to be removed from final file) # Benchmarking single cell RNA-sequencing analysis pipelines using mixture control experiments (Nature) ### Supplementary Figure 3 Visualization of representative benchmarking datasets using t-SNE and UMAP and violin plot of the number of doublets. (a) t-SNE and UMAP visualizations of 4 datasets. From left to right: 10X single cell using 3 cell lines; 10X single cell using 5 cell lines; a CEL-seq2 cell mixture and a CEL-seq2 RNA mixture. Each point represents a cell or ‘pseudo cell’ and the number of cells in each plot is indicated in the title. (b) Violin plot of doublets in each dataset, identified using Demuxlet (DBL: doublet, SNG: single cell). The number of single cells and doublets are shown on top of each violin. Doublets were excluded when calculating the performance metrics. 
Supplementary Figure 5: Example clustering results and summary of clustering performance using ARI and the number of clusters.
- Examples of clustering results visualized by PCA (top) and t-SNE (bottom), with different colours representing the cluster assignments made by the selected method. The sample sizes are 340 for RNAmix_CEL-seq2, 285 for cellmix3 and 274 for sc_CEL-seq2. Coefficients obtained from linear models fitted using the ARI (b) or the number of clusters (c) as dependent variables, and experimental design, normalization methods, imputation methods and clustering methods as covariates. The coefficients measure whether particular features have positive or negative associations with the dependent variables.
The data are from (github) single cell mixology: single cell RNA-seq benchmarking
Install and load awst
if (!requireNamespace("BiocManager", quietly = TRUE))
install.packages("BiocManager")
BiocManager::install("drisso/awst")
#BiocManager::install("GIS-SP-Group/RCA")
#BiocManager::install("hgu133plus2.db")Setup
library(awst)
library(EDASeq)
library(SingleCellExperiment)
jobName <- "mixoloy20200904"
source(url("https://raw.githubusercontent.com/drisso/awst_analysis/master/functions.R"))
true_number_of_clusters <- c(3, 5, 7, 10, 10)
names(true_number_of_clusters) <- c("sc_10x", "sc_10x_5cl", "RNAmix_celseq2", "cellmix4", "cellmix3")
####
results <- matrix(NA, ncol = 5, nrow = 21)
colnames(results) <- c("where", "what", "silhouette", "NDE", "R2")
k <- 1
save_plots <- FALSE
compute_r2 <- function(y, labs) {
if(all(y==0)) {
return(NA)
}
fit <- lm(y ~ labs)
res <- summary(fit)
return(res$r.squared)
}
get_r2 <- function(datamat, labs) {
r2 <- apply(datamat, 2, compute_r2, labs)
return(r2)
}
compute_de <- function(y, labs) {
if(all(y==0)) {
return(1)
}
fit <- lm(y ~ labs)
res <- anova(fit)
return(res[1,5])
}
get_de <- function(datamat, labs) {
pvals <- apply(datamat, 2, compute_de, labs)
pvals <- sort(pvals)
return(datamat[,names(pvals)[1:500]])
}sc_10x_5cl
prefix <- "sc_10x_5cl"con <- gzcon(url("https://github.com/LuyiTian/sc_mixology/blob/master/data/csv/sc_10x_5cl.metadata.csv.gz?raw=true"))
annotation.df <- read.csv(textConnection(readLines(con)))
annotation.df$cell.col <- factor(annotation.df$cell_line)
levels(annotation.df$cell.col) <- c("gold", "red", "blue", "magenta", "green3")
annotation.df$cell.col <- as.character(annotation.df$cell.col)
annotation.df$cell_line <- paste0("mix", annotation.df$mix)
con <- gzcon(url("https://github.com/LuyiTian/sc_mixology/blob/master/data/csv/sc_10x_5cl.count.csv.gz?raw=true"))
ddata <- read.csv(textConnection(readLines(con)))
both <- intersect(colnames(ddata), rownames(annotation.df))
ddata <- ddata[, both]
annotation.df <- annotation.df[both,]
prefix <- "sc_10x_5cl"
save(ddata, annotation.df, prefix, file = paste0(prefix, "_counts.RData"))library(awst)
library(EDASeq)
library(SingleCellExperiment)
load(paste0(prefix, "_counts.RData"))
no_of_detected_gene_per_sample <- colSums(ddata > 0)
fivenum(no_of_detected_gene_per_sample)
ddata <- EDASeq::betweenLaneNormalization(as.matrix(ddata), which = "full", round = FALSE)
# apply the AWS-transformation
tmp <- rowSums(ddata)
sum(tmp > 0)
tmp <- colSums(ddata)
sum(tmp > 0)
exprData <- awst(ddata, poscount = TRUE, full_quantile = TRUE)
sum(is.na(rowSums(exprData)))
sum(is.na(colSums(exprData)))
save(exprData, prefix, file = paste0(prefix, "_expression.RData"))
nrow_exprData <- nrow(exprData)
ncol_exprData <- ncol(exprData)
# library(irlba)
# pprcomp <- prcomp_irlba(exprData, 5) # Run PC analysis
#
# set.seed(2020)
# ans_Rtsne <- Rtsne(exprData, pca = FALSE) # Run TSNE
#
# set.seed(2020)
# ans_umap <- umap(exprData) # Run Umap
#
# save(nrow_exprData, ncol_exprData, annotation.df,
# pprcomp, ans_Rtsne, ans_umap, prefix,
# file = paste0(prefix, "_expression_working.RData"))
save(nrow_exprData, ncol_exprData, annotation.df, prefix,
file = paste0(prefix, "_expression_working.RData"))load(paste0(prefix, "_counts.RData"))
load(paste0(prefix, "_expression.RData"))
# get random subset (to be removed)
set.seed(135)
cellidx <- sample(colnames(ddata), 100)
ddata <- ddata[,cellidx]
annotation.df <- annotation.df[cellidx,]
# awst (exprData)
system.time({
fq <- EDASeq::betweenLaneNormalization(as.matrix(ddata), which = "full", round = FALSE)
exprData <- awst(fq, poscount = TRUE, full_quantile = TRUE)
})user system elapsed 0.223 0.020 0.242
exprData_gf <- awst::gene_filter(exprData, heterogeneity_threshold = .1)
dim(exprData_gf)[1] 100 6068
# VST
library(DESeq2)
get_hvg <- function(mat, k=1000) {
vars <- matrixStats::rowVars(mat)
names(vars) <- rownames(mat)
vars <- sort(vars, decreasing = TRUE)
mat <- mat[names(vars)[seq_len(k)],]
return(mat)
}
dds <- DESeqDataSetFromMatrix(ddata, colData = annotation.df,
design = ~1)
# dds <- estimateSizeFactors(dds)
# dds <- estimateDispersions(dds)
system.time({
vst <- varianceStabilizingTransformation(dds, blind=FALSE)
vst <- assay(vst)
vst1000 <- get_hvg(vst, k=1000)
vst2500 <- get_hvg(vst, k=2500)
vst5000 <- get_hvg(vst, k=5000)
})user system elapsed 10.348 6.587 7.013
system.time({
# rlog
rld <- rlog(dds, blind=FALSE)
rld <- assay(rld)
rld1000 <- get_hvg(rld, k=1000)
rld2500 <- get_hvg(rld, k=2500)
rld5000 <- get_hvg(rld, k=5000)
})## rlog() may take a long time with 50 or more samples,
## vst() is a much faster transformationuser system elapsed 2694.783 4084.403 292.784
# Townes
library(scry)
library(SingleCellExperiment)
system.time({
sce <- SingleCellExperiment(assays = list(counts = ddata),
colData = annotation.df)
sce <- nullResiduals(sce, assay="counts", type="deviance")
sce <- nullResiduals(sce, assay="counts", type="pearson")
res_deviance <- assay(sce, "binomial_deviance_residuals")
# res_deviance <- get_hvg(res_deviance)
res_pearson <- assay(sce, "binomial_pearson_residuals")
# res_pearson <- get_hvg(res_deviance)
})user system elapsed 2.339 4.259 0.346
# CPM
system.time({
cpm <- t(get_CPM(ddata))
cpm1000 <- get_hvg(cpm, k=1000)
cpm2500 <- get_hvg(cpm, k=2500)
cpm5000 <- get_hvg(cpm, k=5000)
})user system elapsed 0.062 0.000 0.062
# raw
raw <- as.matrix(ddata)
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
sc_10x
user system elapsed 0.335 0.008 0.343 [1] 100 15028 user system elapsed 13.720 9.499 9.487
## rlog() may take a long time with 50 or more samples,
## vst() is a much faster transformationuser system elapsed 3933.004 5974.970 429.755 user system elapsed 2.831 4.870 0.404 user system elapsed 0.088 0.000 0.087 
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
RNAmix_celseq2
user system elapsed 0.290 0.002 0.292 [1] 100 6228
## -- note: fitType='parametric', but the dispersion trend was not well captured by the
## function: y = a/x + b, and a local regression fit was automatically substituted.
## specify fitType='local' or 'mean' to avoid this message next time.user system elapsed 21.951 24.092 10.322
## rlog() may take a long time with 50 or more samples,
## vst() is a much faster transformation
## -- note: fitType='parametric', but the dispersion trend was not well captured by the
## function: y = a/x + b, and a local regression fit was automatically substituted.
## specify fitType='local' or 'mean' to avoid this message next time.user system elapsed 2789.856 4126.596 301.494 user system elapsed 2.683 4.702 0.368 user system elapsed 0.079 0.003 0.082 
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
## Warning in anova.lm(fit): ANOVA F-tests on an essentially perfect fit are
## unreliable
Session info
sessionInfo()## R version 4.0.0 (2020-04-24)
## Platform: x86_64-pc-linux-gnu (64-bit)
## Running under: Ubuntu 18.04.4 LTS
##
## Matrix products: default
## BLAS/LAPACK: /usr/lib/x86_64-linux-gnu/libopenblasp-r0.2.20.so
##
## locale:
## [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
## [3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
## [5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=C
## [7] LC_PAPER=en_US.UTF-8 LC_NAME=C
## [9] LC_ADDRESS=C LC_TELEPHONE=C
## [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
##
## attached base packages:
## [1] stats4 parallel stats graphics grDevices utils datasets
## [8] methods base
##
## other attached packages:
## [1] cluster_2.1.0 scry_1.0.1
## [3] DESeq2_1.28.1 SingleCellExperiment_1.10.1
## [5] EDASeq_2.22.0 ShortRead_1.46.0
## [7] GenomicAlignments_1.24.0 SummarizedExperiment_1.18.2
## [9] DelayedArray_0.14.1 matrixStats_0.56.0
## [11] Rsamtools_2.4.0 GenomicRanges_1.40.0
## [13] GenomeInfoDb_1.24.2 Biostrings_2.56.0
## [15] XVector_0.28.0 IRanges_2.22.2
## [17] S4Vectors_0.26.1 BiocParallel_1.22.0
## [19] Biobase_2.48.0 BiocGenerics_0.34.0
## [21] awst_0.0.4 knitr_1.28
## [23] BiocManager_1.30.10
##
## loaded via a namespace (and not attached):
## [1] bitops_1.0-6 bit64_0.9-7 RColorBrewer_1.1-2
## [4] progress_1.2.2 httr_1.4.1 tools_4.0.0
## [7] R6_2.4.1 colorspace_1.4-1 DBI_1.1.0
## [10] tidyselect_1.1.0 prettyunits_1.1.1 bit_1.1-15.2
## [13] curl_4.3 compiler_4.0.0 rtracklayer_1.48.0
## [16] scales_1.1.1 genefilter_1.70.0 askpass_1.1
## [19] rappdirs_0.3.1 DESeq_1.39.0 stringr_1.4.0
## [22] digest_0.6.25 rmarkdown_2.2 R.utils_2.9.2
## [25] jpeg_0.1-8.1 pkgconfig_2.0.3 htmltools_0.4.0
## [28] umap_0.2.5.0 dbplyr_1.4.4 rlang_0.4.6
## [31] RSQLite_2.2.0 generics_0.0.2 jsonlite_1.6.1
## [34] hwriter_1.3.2 dplyr_1.0.0 R.oo_1.23.0
## [37] RCurl_1.98-1.2 magrittr_1.5 GenomeInfoDbData_1.2.3
## [40] Matrix_1.2-18 munsell_0.5.0 Rcpp_1.0.4.6
## [43] reticulate_1.16 lifecycle_0.2.0 R.methodsS3_1.8.0
## [46] stringi_1.4.6 yaml_2.2.1 MASS_7.3-51.6
## [49] zlibbioc_1.34.0 BiocFileCache_1.12.1 grid_4.0.0
## [52] blob_1.2.1 crayon_1.3.4 lattice_0.20-41
## [55] splines_4.0.0 GenomicFeatures_1.40.1 annotate_1.66.0
## [58] hms_0.5.3 locfit_1.5-9.4 pillar_1.4.4
## [61] geneplotter_1.66.0 biomaRt_2.44.1 XML_3.99-0.3
## [64] glue_1.4.1 evaluate_0.14 latticeExtra_0.6-29
## [67] png_0.1-7 vctrs_0.3.1 gtable_0.3.0
## [70] openssl_1.4.1 purrr_0.3.4 assertthat_0.2.1
## [73] ggplot2_3.3.1 xfun_0.14 aroma.light_3.18.0
## [76] xtable_1.8-4 RSpectra_0.16-0 survival_3.1-12
## [79] glmpca_0.2.0 tibble_3.0.1 AnnotationDbi_1.50.3
## [82] memoise_1.1.0 ellipsis_0.3.1Table
| where | what | silhouette | NDE | R2 |
|---|---|---|---|---|
| raw | sc_10x_5cl | 0.0666 | 500 | 0.5448 |
| cpm | sc_10x_5cl | 0.0462 | 500 | 0.5693 |
| awst | sc_10x_5cl | 0.0990 | 500 | 0.5141 |
| vst | sc_10x_5cl | 0.0731 | 500 | 0.5983 |
| rlog | sc_10x_5cl | 0.1427 | 500 | 0.6014 |
| deviance | sc_10x_5cl | 0.1370 | 500 | 0.5906 |
| pearson | sc_10x_5cl | 0.1232 | 500 | 0.5546 |
| raw | sc_10x | 0.1194 | 500 | 0.6635 |
| cpm | sc_10x | 0.0734 | 500 | 0.6840 |
| awst | sc_10x | 0.1286 | 500 | 0.6588 |
| vst | sc_10x | 0.1207 | 500 | 0.6984 |
| rlog | sc_10x | 0.1567 | 500 | 0.7015 |
| deviance | sc_10x | 0.2050 | 500 | 0.6724 |
| pearson | sc_10x | 0.1677 | 500 | 0.6251 |
| raw | RNAmix_celseq2 | -0.0415 | 500 | 0.3194 |
| cpm | RNAmix_celseq2 | -0.0151 | 500 | 0.3718 |
| awst | RNAmix_celseq2 | -0.0083 | 500 | 0.3458 |
| vst | RNAmix_celseq2 | -0.0184 | 500 | 0.4259 |
| rlog | RNAmix_celseq2 | 0.0118 | 500 | 0.4378 |
| deviance | RNAmix_celseq2 | 0.0093 | 500 | 0.4376 |
| pearson | RNAmix_celseq2 | 0.0086 | 500 | 0.4343 |
knitr::knit_exit()